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. Author manuscript; available in PMC: 2009 Jul 16.
Published in final edited form as: Am J Physiol Heart Circ Physiol. 2005 Jan 6;288(5):H2077–H2087. doi: 10.1152/ajpheart.00526.2003

Figure 7.

Figure 7

Representative Western blots together with summary data for K channel subunits. A. Protein levels of putative Ito subunits. Antibodies to Kv4.2 exhibit a single major band at 70 kDa which was significantly decreased in failing hearts (Nc=6; Nf=5, p < 0.05). Two different anti-Kv4.3 antibodies were used, an antibody that recognizes total Kv4.3 exhibits two major bands at 78 and 68 kDa that were quantified (Nc=6; Nf=6; p = NS). The primary antibody was specific for the long splice variant, anti-Kv4.3L, recognized a single major band at 78 kDa that was significantly reduced in failing ventricles (Nc=8; Nf=8; p = 0.0003). Anti-Kv1.4 antibodies recognize a single band at 96 kDa that was unchanged in the failing hearts (Nc=12; Nf=10, p = NS). Two anti-KChIP antibodies were used, an antibody specific for KChIP2S/T isoforms reveals two bands at 25 and 26 kDa that were unchanged in the failing heart (Nc=4; Nf=8, p = NS). A pan-KChIP antibody recognizes a single major band at 34 kDa that was significantly decreased in the failing myocardium (Nc=7; Nf=7, p < 0.05). B. Delayed rectifier immunoreactive protein. Single bands at ∼70 kDa were recognized with antibodies specific for KvLQT1 (Nc=10; Nf=11; p = NS), there was no significant change in failing hearts compared with controls. C. The anti-Kir2.1 antibody recognizes one band at 55 kDa that was unchanged in the failing hearts (Nc=6; Nf=8, p = NS). AU: Arbitrary Units.