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. Author manuscript; available in PMC: 2009 Jul 16.
Published in final edited form as: Cell. 1991 Jul 12;66(1):173–183. doi: 10.1016/0092-8674(91)90149-s

Figure 1. Stimulation of [3H]Thymidine Incorporation by NGF.

Figure 1

(A) Quiescent NIH 3T3, E25-48, and B35-41 cells were incubated with DMEM containing 5 μg/ml insulin (open bars), 20% calf serum (hatched bars), or 30 ng/ml NGF plus 5 μg/ml insulin (filled bars). Results are expressed as the mean of three independent experiments assayed in duplicate (+SEM).

(B) E25-43 cells were stimulated by the indicated amounts of NGF either in the absence (open circles) or presence (filled circles) of a 10-fold molar excess of anti-NGF monoclonal antibody clone 27/21 (Boehringer Mannheim).