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. Author manuscript; available in PMC: 2009 Jul 16.
Published in final edited form as: Cell. 1991 Jul 12;66(1):173–183. doi: 10.1016/0092-8674(91)90149-s

Figure 3. Responsiveness to NGF Correlates with Expression of the trk Proto-Oncogene Product.

Figure 3

E25-48 cells were assayed for BrdUrd incorporation as described in the legend to Figure 2 and the results photographically recorded. Microslides containing BrdUrd-stained E25-48 cells were then used to immunocytochemically visualize gp140trk expression using the avidin–biotin immunoperoxidase technique. (A) Preimmune rabbit serum. (B, C) Rabbit polyclonal anti-trk antiserum. (D) BrdUrd incorporation in the same field as shown in (C). Final magnifications: 270x (A, B) and 400x (C, D). Asterisks indicate cells without detectable gp140trk expression (C) and without BrdUrd incorporation (D).