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. Author manuscript; available in PMC: 2009 Jul 16.
Published in final edited form as: Cell. 1991 Jul 12;66(1):173–183. doi: 10.1016/0092-8674(91)90149-s

Figure 4. NT-3 Competes with 125I-Labeled NGF for Binding to the trk Receptors Expressed in E25-48 Cells.

Figure 4

E25-48 cells were preincubated with unlabeled NGF (circles), NT-3 (squares), or BDNF (triangles) at 4°C for 1 hr. 125I-labeled NGF was added to the cultures at a final concentration of 5 ng/ml, and the cells were incubated for an additional 2 hr. The percentage of 125I-labeled NGF bound to the cells was determined as described in Experimental Procedures. Results are expressed as the mean of duplicate samples.