Figure 5. NT-3 Induces DNA Synthesis in E25-48 Cells.
The mitogenic activity of NT-3 was measured by immunofluorescence after BrdUrd incorporation. Cells were stimulated with DMEM containing 20% calf serum (A), 5 μg/ml insulin (B), or 50 ng/ml NT-3 plus 5 μg/ml insulin (C). Final magnification 250x.