Table 2.
Growth in Semisolid Medium of Cells Expressing the gp140trk Receptors
Cell Line | Number of Cells | Additionsa | Agar Coloniesb | NGF Stimulation |
---|---|---|---|---|
NIH 3T3 | 103 | – | 1 | |
103 | NGF | 0 | None | |
104 | – | 1 | ||
104 | NGF | 2 | None | |
E25-48 | 103 | – | 12 | |
103 | NGF | 111 | 9-fold | |
104 | – | 79 | ||
104 | NGF | 546 | 7-fold | |
B38-94 | 103 | – | 98 | |
103 | NGF | 90 | None | |
104 | – | 1604 | ||
104 | NGF | 1356 | None |
Cultures were fed every 4 days with 0.5 ml of DMEM containing 5% calf serum in the absence or presence of 30 ng/ml NGF as indicated.
Colonies containing more than 32 cells were scored as positive after 4 weeks of culture.