Table 5.
Transformation of NIH 3T3 Cells by Cotransfection of Expression Plasmids Encoding gp140trk, NGF, and NT-3
| Cotransfected DNAs | ||||||
|---|---|---|---|---|---|---|
| gp140trk Receptors |
Neurotrophic Factors |
Transforming Activity (Foci per 1.5 × 105 Cells) |
||||
| Plasmid | DNA (ng) | Plasmida | DNA (ng) | Exp. 1 | Exp. 2 | |
| pDM69 | 2000 | – | – | 0 | 0 | |
| – | – | pLTRSNGF (NGF) | 2000 | 0 | 0 | |
| pDM69 | 2000 | + | pLTRSNGF (NGF) | 2000 | TMTCb | TMTCb |
| pDM69 | 2000 | + | pLTRSNGF (NGF) | 200 | TMTC | TMTC |
| pDM69 | 2000 | + | pLTRSNGF (NGF) | 20 | 350 | 344 |
| pDM69 | 2000 | + | pLTRSNGF (NGF) | 2 | 53 | 32 |
| – | – | pLL43 (NT-3) | 2000 | 0 | 0 | |
| pDM69 | 2000 | + | pLL43 (NT-3) | 2000 | 380 | 210 |
| pDM69 | 2000 | + | pLL43 (NT-3) | 200 | 78 | 44 |
| pDM69 | 2000 | + | pLL43 (NT-3) | 20 | 6 | NDc |
| pDM69 | 2000 | + | pLL43 (NT-3) | 2 | 0 | ND |
| – | – | pLL42 (BDNF) | 2000 | 0 | 0 | |
| pDM69 | 2000 | + | pLL42 (BDNF) | 2000 | 0 | 0 |
| pDM69 | 2000 | + | pLL42 (BDNF) | 200 | 0 | 0 |
a
The neutrophic factor encoded by each plasmid is indicated in parenthesis.
b
TMTC, too many to count.
c
ND, not done.