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. Author manuscript; available in PMC: 2009 Jul 16.
Published in final edited form as: J Biol Chem. 2006 May 11;281(28):19688–19699. doi: 10.1074/jbc.M601110200

FIGURE 5. β8 expression down-regulates RhoA activity.

FIGURE 5

MCs from itgb8+/+ and itgb8−/− mice were harvested and maintained in primary culture. A, to verify itgb8 gene deletion, whole cell lysates were probed for β8 integrin protein expression by immunoblot analysis. B, itgb8+/+ and itgb8−/− MCs were incubated with poly-l-lysine (PLL, 10 μg/ml, 30 min) or vitronectin (VN, 10 μg/ml, 30 min) and then assayed for Rac1 and RhoA activities, as described in Fig. 4.