Skip to main content
. Author manuscript; available in PMC: 2009 Jul 16.
Published in final edited form as: J Biol Chem. 2006 May 11;281(28):19688–19699. doi: 10.1074/jbc.M601110200

FIGURE 6. β8 activation is associated with Rac1 release from GDI.

FIGURE 6

HEK293 cells expressing IL-2RΔ, IL-2R/β8, IL-2R/β3, or empty vector were incubated with clustering Tac or control antibodies. Chimeric integrins were immunoprecipitated (IP) from cell lysates with Tac IgG, resolved by SDS-PAGE, and then probed for GDI or Rac1 by immunoblot analysis (upper two panels). Fractions of cell lysate before immunoprecipitation were immunoblotted with anti-GDI and anti-Rac1 antibodies (lower two panels) to assess expression.