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. Author manuscript; available in PMC: 2009 Aug 21.
Published in final edited form as: Brain Res. 2008 Jun 14;1226:18–26. doi: 10.1016/j.brainres.2008.06.013

Figure 2.

Figure 2

Attenuation and over-expression of CXCR4 by vectors carrying siRNA-CXCR4 and CXCR4 cDNA, respectively. Panel A shows approximately 90% of the adult neural progenitor cells exhibited GFP signals 24h after transfection with siRNA-CXCR4 (A, GFP) compared with the total number of cells under the bright field (A, bright). Over-expression of CXCR4 in adult neural progenitor cells increased CXCR4 mRNA (B, CXCR4) and protein (C, CXCR4). In contrast, transfection with three siRNA-CXCR4 vectors resulted in substantial reduction of endogenous mRNA levels with a maximum blockage of 80% mRNA by the pSIREN-RetroQ-siRNA-CXCR4-1 vector (B and D). Panel E shows siRNA-CXCR4 suppressed CXCR4 protein on Western blots. Beta-actin was used as an internal control. *p<0.05, N=3/group. Scale bar: 100µm.