DCs express ST2 mRNA and ST2 protein. A, Total RNA was purified from MACS® purified DCs (>93% CD11c+ cells) (n=3) and from flow-through (23% CD11c+ cells) populations (n=3). ST2 mRNA was quantified by real-time RT-PCR. B, Gel electrophoresis comparison of amplified PCR products: CD11c+ denotes purified DCs, FT denotes flow-through DCs. C, FACS analyses of purified DCs show surface and intracellular expression of ST2 protein; control Ab is rat IgG1 isotype control (dashed line), no stain (shaded area), anti-ST2 (solid line).