Figure 1.

Verification of transgene integration by Southern blot analysis. Genomic DNA from the nontransformed and transformed mosquito lines was digested with EcoRI and BamHI and analyzed on a Southern blot with a 1.6-kb luciferase probe. The predicted 2.0-kb luciferase fragment is observed in five of the six AeCP transgenic lines and in the AgCP line but not in the nontransformed line. To verify loading of DNA in all lanes, the blot subsequently was hybridized with a late trypsin probe (loading control). The source of DNA is indicated at the top of each lane. NT: DNA from kh^w/kh^w (nontransformed) mosquitoes. Numbers followed by a T indicate the designation of each transformed mosquito line. AgCP: DNA from mosquitoes transformed with the AgCP construct.