Figure 2.

Assessment of AeCP expression by RT-PCR. RT-PCR was conducted with a mixture of two sets of primers: one specific for luciferase that generates a 376-nt fragment (luciferase), and one specific for the endogenous carboxypeptidase that generates a 237-nt fragment (AeCP). The template was total RNA extracted from guts 24 h after a blood meal. Luciferase mRNA was detected in five of the six transgenic lines. No luciferase signal was detected with RNA from nontransformed mosquitoes (NT), as expected. The source of RNA is indicated at the top of each lane (see Fig. 1 legend). DNA size markers were run in the first lane of each panel. +RT: PCRs done after RT of the RNA; −RT: RT was omitted.