Figure 4.

Analysis of transgenic mRNA accumulation. (A) Time course of luciferase mRNA accumulation after a blood meal. Total RNA extracted from guts (or carcasses) dissected at different times after a blood meal was fractionated by electrophoresis on agarose gels and blotted to a nylon membrane. The membrane was first hybridized with a radioactive luciferase DNA probe and then with a mitochondrial rRNA probe, used as a loading control. Numbers above the lanes refer to times after a blood meal when AeCP transgenic mosquitoes were dissected. 0: Guts from sugar-fed mosquitoes; C: carcasses (nongut tissues); NT: guts from nontransformed mosquitoes dissected 24 h after a blood meal; M: male gut. (B) Assessment by Northern blot analysis of luciferase expression in mosquitoes transformed with the AgCP construct. RNA was extracted from different tissues, developmental stages, or sexes and analyzed by Northern blotting with a radioactive luciferase probe. RNA loading was verified as above. G: Guts (numbers indicate hours after a blood meal); C: carcass; LC: larval carcass; LG: larval gut; Pp; pupae; M: male gut.