Fig. 1.

Superoxide anion production (A) and degranulation (B) by human eosinophils stimulated with ESP produced by PwNEM. Eosinophils were stimulated with three different concentrations (10, 30 and 100 µg/ml) of ESP, PAF (1 µM), or medium alone, for up to 3 hr at 37℃. Superoxide production was then measured by determining the superoxide dismutase-inhibitable reduction of cytochrome c. After 3 hr of incubation, the concentrations of EDN in the cell-free supernatants were measured by RIA, as an index of eosinophil degranulation. Data are expressed as means ± SEM from three independent experiments with different eosinophil donors. ^*, denotes significant differences (P < 0.05) as compared with the medium alone.