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Phylogenetic tree of Acanthamoeba 20 isolates based on 18S rDNA sequences. The scale bar represents an evolutionary distance equivalent to 1%. The species names of six reference strains are presented in Table 2. The others are A. castellanii Ma, A. castellanii Neff, A. lugdunensis L3a, A. triangularis SH621, A. stevensoni RB:F:1, A. hatchetti BH-2, A. griffini S-7, A. pustulosa GE3a, A. lenticulata PD2S, and A. culbertsoni Lilly.
