Solution structure of Rsn-2. (A) Ribbon diagram illustrating the Rsn-2 fold and (B) rotated 90° about the vertical axis. Residues showing conformational heterogeneity in the NMR spectra are indicated in magenta. (C) Stereo-pair superposition of the backbone structures of the 25 lowest-energy NMR structures. (D–G) Hydrophobic surface maps of the entire Rsn-2 molecule, front face (D) and back face (G), with the inner face of the helical segment (E) and the inner face of the sheet segment (F). Color codes: white, hydrophobic; red, negative; blue, positive; and yellow, sulfur. Figures prepared using PyMOL (DeLano Scientific, San Francisco, CA).