Table 1.

Best-fit neutron reflection parameters for two-layer surface adsorption of Rsn-2 in D₂O or NRW

	Solvent	Subphase concentration/mg mL−1	Layer thickness τ/Å	ρobs∗/10−6	ρp†/10−6	Volume fraction‡ϕp	Surface excess/mg m−2	Area per molecule/Å2
Layer 1	D2O	0.007	8	3.0	3.23	0.93	1.02	1782
	NRW	0.007	8	1.8	1.92	0.94	1.03	1765
	D2O	0.05	8	3.0	3.23	0.93	1.02	1781
	NRW	0.05	8	1.8	1.92	0.94	1.03	1765
	D2O	0.25	10	3.0	3.23	0.93	1.28	1425
	NRW	0.25	10	1.8	1.92	0.94	1.29	1412
Layer 2	D2O	0.007	20	6.0	3.23	0.11	0.31	5900
	NRW	0.007	16	0.3	1.92	0.16	0.34	5295
	D2O	0.05	30	6.0	3.23	0.11	0.46	3933
	NRW	0.05	25	0.4	1.92	0.21	0.72	2541
	D2O	0.25	40	5.6	3.23	0.24	1.32	1377
	NRW	0.25	35	0.6	1.92	0.31	1.50	1210

^∗Neutron scattering length density from experimental fits.

^†Calculated from amino acid sequence data, assuming appropriate H/D exchange.

^‡Calculated using ρ_obs = ϕ_pρ_p for layer 1 (in air) and ρ_obs = ϕ_pρ_p + ϕ_wρ_w for layer 2 (in water).