Table 1.
Domain | CM AFM fixed neuron |
CM AFM live neuron |
DM AFM live neuron |
||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Average height (nm) | SD∗ (nm) | n† | Corrected height‡ (nm) | Average height (nm) | SD∗ (nm) | n† | Corrected height‡ (nm) | Average height (nm) | SD∗ (nm) | n† | |
P lamellipodia | 53 | 24 | 82 | 122 | 107 | 33 | 41 | 183 | 142 | 28 | 77 |
P filopodia | 73 | 11 | 24 | — | 149 | 25 | 76 | 217 | 175 | 16 | 85 |
T zone ruffles | 577 | 175 | 12 | 623 | 589 | 274 | 21 | 690 | 848 | 235 | 23 |
C domain | 1020 | 191 | 10 | 1113 | 1137 | 164 | 13 | 1322 | 1310 | 137 | 11 |
AFM, atomic force microscope; C, central; CM, contact mode; DM, dynamic mode; P, peripheral; SD, standard deviation; T, transition.
SD of the heights of individual growth cone domains as measured with AFM.
Number of measurements.
Corrected height was calculated by adding the average indentation of each growth cone domain to the average height. The variations in the corrected domain heights are equal to or greater than that of the average measured heights. Note that the corrected heights for fixed neurons are different from our previous report (38) due to a revised analysis of the indentation data.