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Comparison of melibiose-induced difference spectra of R141C and Cys-less in the presence of Na+. (A) Solid line: Difference spectrum of R141C in 20 mM MES, 100 mM KCl, 10 mM NaCl, 10 mM melibiose, pH 6.6, minus R141C in 20 mM MES, 100 mM KCl, 10 mM NaCl, pH 6.6; shaded line: difference spectrum of MelB Cys-less under the same conditions. (B) Deconvoluted difference spectra of panel A.
