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Confocal microscopy image of 5-IAF-labeled profilin binding on a GUV made of 99% POPC and 1% unlabeled PI(4,5)P2. (A) Intensity map of the GUV membrane. (B) The highlighted pixels correspond to a molecular brightness of 1.11. (C) The highlighted pixels are clusters of profilin and correspond to a molecular brightness of 1.23. (D) Brightness map of the same GUV membrane.
