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Confocal microscopy images of GUVs. (A) 5-IAF-labeled profilin in the presence of a GUV made of 100% POPC (average of 100 frames). (B–F) GUV containing 99% POPC and 1% BODIPY TMR-labeled PI(4,5)P2 in the presence of profilin. (D) Shmoo-like deformation of the GUV. (B) Budding of the GUV and the presence of lipid inclusions. (C) Altered GUV after several hours in the presence of profilin. (E and F) GUVs grown in the presence of 10 μM profilin.
