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Specificity constants (kcat/KM) of the reaction catalyzed by E. coli IPMDH for NAD+ (A) and IPM (B) as a function of temperature. The specificity constant kcat/KM,IPM has an unusual temperature dependence, having a local minimum at ∼35°C. The specificity constant kcat/KM,NAD has no unusual features. Error bars represent standard errors obtained as described in Materials and Methods. The solid lines are visual guides to the eye.
