Figure 5.

Temperature dependence of enthalpy (■), entropy (♦), and Gibbs free energy (○) of the binding of IPM to IPMDH measured by ITC. The data were analyzed by assuming a 1:1 binding stoichiometry using MicroCal Origin 5.0 software. Standard deviation was calculated according to the fit by Origin. Inset: The temperature dependence of heat capacity change associated with substrate binding was calculated from the temperature dependence of binding enthalpy. The solid lines are visual guides to the eye.