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. 2009 Jul 31;5(7):e1000533. doi: 10.1371/journal.ppat.1000533

Figure 1. PSM-mec production in S. aureus and S. epidermidis.

Figure 1

(A) RP-HPLC elution profile of S. aureus MSA3407 and S. epidermidis RP62A culture filtrates (at 8 h of growth). Equal volumes were applied to a C8 column and eluted with a 0.1% TFA water/acetonitrile gradient. PSM peptides characteristically elute at very high acetonitrile concentrations. Peaks of major PSM peptides and the newly identified PSM-mec are indicated. (B) Electrospray ion chromatogram of the PSM-mec peak obtained by RP/HPLC/ESI-MS (S. epidermidis RP62A). The respective PSM-mec ESI chromatogram of S. aureus MSA3407 or any strain with PSM-mec production showed the same m/z peaks. The series of peaks with slightly higher masses close to m/z 1208.3 are due to water and sodium adducts.