Figure 2.

Effect of p40₂ and p70 on the expression of lymphotoxin-α (Lt-α) messenger RNA in different cell types. Microglia isolated from 7- to 9-day-old mouse pups (a), microglia isolated from 13- to 17-week-old human fetal brains (b), mouse peritoneal macrophages (c), RAW 264.7 cells (d), and splenic T cells (e) were stimulated with different concentrations of p40₂ and p70 under serum-free condition. After 6 hr of stimulation, the messenger RNA expression of Lt-α was monitored by semi-quantitative reverse transcription–polymerase chain reaction. Results represent three independent experiments.