Figure 3.

Combinatorial effects of soluble egg antigen (SEA) and lipopolysaccharide (LPS) on regulated gene expression in bone marrow macrophages (BMMs). BMMs were plated overnight in the absence of colony-stimulating factor (CSF)-1, and then treated for 2 hr with 10 ng/ml LPS, 10 μg/ml SEA, both, or medium as a control. Gene expression (mean of triplicates + standard error of the mean) was calculated by real-time polymerase chain reaction (PCR) for Jagged-1 (a), interleukin (IL)-33 (b) and IL-12p40 (c). The results shown are indicative of seven similar experiments.