Figure 4.

Jagged-1 induction in antigen-presenting cells (APCs) does not correlate with permissiveness for T helper type 2 (Th2) responses (a and b). Bone marrow macrophages (BMMs) [plated overnight without colony-stimulating factor (CSF)-1] or dendritic cells (DCs) were treated for 2 hr with 10 ng/ml lipopolysaccharide (LPS), 10 μg/ml soluble egg antigen (SEA) or medium as a control. Gene expression was calculated by real-time polymerase chain reaction (PCR) for Jagged-1 (a) and interleukin (IL)-12p40 (b). (c) BMMs or DCs were stimulated overnight with medium, 10 ng/ml LPS, 10 μg/ml SEA or 0·1 μm CpG DNA, and then co-cultured with purified mouse splenocytes for 7 days, followed by anti-CD3 stimulation in combination with a second treatment of medium, LPS, SEA or CpG DNA for 3 days. IL-4 levels in cell culture supernatants were determined by enzyme-linked immunosorbent assay (ELISA). Data are representative of three or more similar experiments.