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. 2009 Jul;127(3):326–337. doi: 10.1111/j.1365-2567.2008.02979.x

Figure 5.

Figure 5

Soluble egg antigen (SEA) signals in HEK293 cells expressing Toll-like receptor 2 (TLR2) or TLR4/MD2, but not TLR9. HEK293 cells expressing TLR9 (a), TLR3 (b), TLR2 (c) or TLR4/MD2 (d) were treated for 24 hr with 10 μg/ml SEA, TLR-specific positive controls (10 ng/ml LPS, 0·1 μg/ml CpG DNA (2006), 30 μg/ml PolyIC and 20 μg/ml zymosan) and vehicle controls. Interleukin (IL)-8 production in culture supernatants was determined by enzyme-linked immunosorbent assay (ELISA). Results from three independent experiments (except for HEK293 cells expressing TLR3, which represents four independent experiments) are displayed as fold induction (relative to unstimulated cells) + standard error of the mean.