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. 2009 May 27;29(21):7003–7014. doi: 10.1523/JNEUROSCI.1110-09.2009

Figure 5.

Figure 5.

STAT3 is a potential transcriptional regulator in the promoter of the mouse Mn-SOD gene. A, Schematic diagram for putative STAT3 binding sites in the promoter of the mouse Mn-SOD gene. B, Transcriptional activity of the mouse Mn-SOD gene was determined by luciferase assay. The HEK293T cells transfected with pGLu–Mn-SOD luciferase construct were treated with 50 μm AG490 and 50 μm JSI-124 for 24 h and evaluated by luciferase activity. Data are mean ± SEM. *p < 0.001, #p < 0.05 versus control. C, Transcriptional activity of the mouse Mn-SOD gene in response to changes in STAT3 activity was determined by luciferase assay in HEK293T cells transfected with STAT3-specific siRNA. Data are mean ± SEM. *p < 0.001, #p < 0.05 versus control. D, Transcriptional activity of the mouse Mn-SOD gene in response to changes in STAT3 activity was determined by luciferase assay in primary cortical neurons transfected with STAT3-specific siRNA or treated with 50 μm AG490. Data are mean ± SEM. *p < 0.001, #p < 0.05 versus control. E, Western blot analysis of the protein level of total STAT3 in HEK293T cells transfected with STAT3-specific siRNA. F, Western blot analysis of the protein level of total STAT3 in primary cortical neurons transfected with STAT3-specific siRNA.