Skip to main content
. 2009 May 27;29(21):7003–7014. doi: 10.1523/JNEUROSCI.1110-09.2009

Figure 6.

Figure 6.

Active STAT3 binds on the putative motifs in the Mn-SOD promoter and its binding is blocked by ischemic reperfusion. A, Activity of STAT3 binding to DNA in the Mn-SOD promoter was determined by EMSA analysis using nuclear extracts from sham-operated mouse brain cortices or from mouse cortices subjected to 3 h of reperfusion after MCAO. Using 12 pairs of the STAT3 putative binding probes, the putative STAT3 binding sites are selected and identified. Using a phospho-STAT3 (Y705) antibody, the supershift (SS) of the STAT3-DNA binding complex was confirmed. B, Quantitative graph depicting the change in STAT3/DNA binding level after ischemic reperfusion relative to shams. *p < 0.05 (n = 3 per group). C, In primary cortical neurons, activity of STAT3 binding to DNA in the Mn-SOD promoter was determined by EMSA analysis using nuclear extracts from cells treated with the vehicle or 50 μm AG490 for 3 h. Using a phospho-STAT3 (Y705) antibody, the super shift of the STAT3-DNA binding complex was confirmed. D, Quantitative graph depicting the change in the STAT3/DNA binding level after STAT3 inhibition relative to control. *p < 0.05 (n = 3 per group). S, Sham; IR, ischemic reperfusion.