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Steady-state kinetic parameters and actin-sliding velocities for wild-type and 15b myosins
| Myosin isoform | Basal MgATPase (s−1 head−1) | Actin-activated Vmax (s−1 head−1) | KM actin (μM) | n | Actin sliding velocity (μm s−1) | n | |
|---|---|---|---|---|---|---|---|
| Wild-type | 0.22 ± 0.01 | 1.70 ± 0.13 | 0.31 ± 0.05 | 11 | 7.4 ± 0.1 | 3 | |
| 15b | 0.17 ± 0.01 | 1.57 ± 0.12 | 0.12 ± 0.04∗ | 5 | 7.5 ± 0.1 | 3 |
Temperature, 22–23°C.
Significant difference (p < 0.05) from wild-type.
