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. 2009 May 20;96(10):4144–4152. doi: 10.1016/j.bpj.2009.02.015

Figure 1.

Figure 1

The unusual ITC-binding curves under a low-salt condition. Sample raw data for the titration of HMGA2 into FL-AT-1 (A) and FL-AT-2 (B) at 25°C in 1 × BPE containing 50 mM of NaCl (total 66 mM Na+). Top: each peak shows the heat produced by injection of an aliquot of 15 μL of HMGA2 (35.7 μM) into DNA solution (1.44 mL of 5 μM). Bottom: the binding isotherm generated from integration with respect of time with appropriate dilution correction.