Table 2.

Comparison of thermodynamic parameters for HMGA2 binding to FL-AT-1 and FL-AT-2

DNA	Kobs	ΔGobs	ΔH	−TΔS	ΔCp
FL-AT-1	1.6 × 107	−9.8	3.4	−13.4	−330
FL-AT-2	2.9 × 107	−10.2	−12.1	1.9	−377

K_obs (M⁻¹) is the binding constant for the interaction of HMGA2 with DNA and refers to solutions containing 0.20 M NaCl at 25°C. ΔG_obs (kcal mol⁻¹) is the binding free energy calculated from the equation ΔG_obs = −RTlnK_obs. The DNA-binding enthalpy ΔH was determined by ITC experiments. −TΔS was calculated by subtraction, −TΔS = ΔG−ΔH.