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Vj-gating in HeLa cell pair forming Cx31-EGFP/Cx45 heterotypic junctions. Ij trace shows strong asymmetry of Ij response to Vj ramps slowly rising from 0 to −115 mV and from 0 to 115 mV. Gj-Vj plot (normalized to maximal gj at Vj = ∼−40 mV) shows that at Vj = 0 only ∼50% of Cx31-EGFP/Cx45 channels are open. Data shown in the inset demonstrate an increase of gj at Vjs >60 mV.
