. 2009 May 20;96(10):4122–4131. doi: 10.1016/j.bpj.2009.02.050

Table 1.

Fusion and hemifusion rate constants and branching fractions from 5 ms/frame movies using dual labeling with R18 and calcein

v-SNARE vesicle % DOPE^∗	N_total^†	Full fusion^‡		Hemifusion^‡
v-SNARE vesicle % DOPE^∗	N_total^†	f_fus	k_fus (s⁻¹)	f_hemi	k_hemi (s⁻¹)	f_core	k_core (s⁻¹)	f_dead-end
0	172	0.95	120 ± 30	0.05	–	–	–	–
30	219	0.82	120 ± 30	0.18	130 ± 30	0.18	10.2 ± 2.5	–
60	200	0.35	220 ± 80	0.65	120 ± 30	0.39	11.8 ± 3.0	0.26

^∗

In addition to DOPE, vesicles contain 5% R18 labels, 15% DOPS, and the remainder POPC.

^†

Total number of events measured.

^‡

See text for classification scheme. Branching fractions are f_fus for prompt, full fusion, and f_hemi for the sum of productive (f_core) and dead-end (f_dead-end) hemifusion. The rate constant k_hemi includes both productive and dead-end hemifusion events; in the former, k_core measures the rate at which hemifusion intermediates decay via core fusion.