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. 2009 May 20;96(10):4063–4074. doi: 10.1016/j.bpj.2009.02.055

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© 2009 by the Biophysical Society.

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Electrophysiological analysis of the homomeric and heteromerized truncated constructs. Current traces recorded in oocytes injected with the full-length construct KDC1 and the truncated constructs KDC1(cNBD) and KDC1(ΔK_HA) (A) and the same constructs coinjected with KAT1(B). (C) Comparison ± Zn²⁺ at −160 mV for KAT1 and KAT1 coinjected with KDC1 or with the truncated KDC1 constructs. (D) Change of the current induced by 1 mM Zn²⁺ at −160 mV (I_zinc-I_control)/I_control for the same constructs of panel C. Data are shown as mean ± SE. The values obtained for the KAT1/KDC1(ΔK_HA) pair were significantly larger than the values obtained for KAT1 (Student's t-test, p < 0.0001), whereas the values obtained for the KAT1/KDC1(cNBD) pair were not (p = 0.77).