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. 2009 May 20;96(10):4063–4074. doi: 10.1016/j.bpj.2009.02.055

Figure 7.

Figure 7

Open probability and kinetics of channels obtained from the coexpression of KAT1 and different KDC1 constructs, either tagged or untagged with GFP. (A) Open probability characteristics of the homomeric KAT1 channel, the heteromeric KAT1/KDC1 channel, and channels obtained coexpressing KAT1 with truncated KDC1 with and without EGFP. Data were obtained from steady state currents like those shown in Fig. 5. Continuous curves are the best fit of the mean open probability (± SE) obtained from at least seven experiments for each construct yielding the following values: KAT1(V1/2 = −119 mV, z = 1.7); KAT1/KDC1(V1/2 = −208 mV, z = 1.3); KAT1/KDC1-EGFP(V1/2 = −163 mV, z = 1.0); KAT1/EGFP-KDC1(V1/2 = −164 mV, z = 1.1); KAT1/KDC1(cNBD) (V1/2 = −120 mV, z = 1.7); KAT1/KDC1(ΔKHA) (V1/2 = −143 mV, z = 1.0); KAT1/KDC1(cNBD)-EGFP(V1/2 = −118 mV, z = 1.6); KAT1/KDC1(ΔKHA)-EGFP (V1/2 = −117 mV, z = 1.3). (B) Activation current traces of the investigated channels were fitted with two exponentials and the activation time constant of the slower exponential, τ2, was plotted versus the applied potential. It can be observed that the activation time constant, τ2, for the construct KAT1/KDC1(ΔKHA) significantly differs from that observed for KAT1 (Student's t-test, ranging from p < 0.002 to p < 0.0001). Data are mean ± SE of at least seven experiments.