a |
Membrane thickness. |
ae
|
Membrane electrical thickness. |
A |
Area of the vesicle. |
Ap
|
Permeabilized area. |
C |
Constant depending on R, a, and the various conductivities of the problem. |
DiIC18
|
1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate. |
DOPC |
1,2-Dioleoyl-sn-glycero-3-phosphocholine. |
EggPC |
L-α-Phosphatidylcholine (egg, chicken). |
E |
Magnitude of the applied electric field. |
l |
Length of the hydrocarbon chain. |
Nc
|
Number of pulses needed to enter the shrinking regime. |
p |
Dipole moment of the PC headgroup. |
PR |
Preshrinking regime. |
q |
Probability that one pulse induces a transition from the pre-shrinking to the shrinking regime. |
Rc
|
Critical radius. |
λ |
Fraction of the permeabilized area lost per pulse. |
Rhodamine PE |
L-α-Phosphatidylethanolamine-n-(Egg Lissamine Rhodamine PE). |
R(n) |
Radius of the vesicle after n pulses. |
Wc
|
Rescaled critical radius, Rc/R(0) |
SR |
Shrinking regime. |
W(n) |
Rescaled radius of the vesicle after n pulses, R(n)/R(0) |
ΔΨ |
Transmembrane voltage. |
ΔΨ0
|
Initial transmembrane voltage induced by the first pulse at the poles of the liposomes. |
ΔΨc
|
Critical transmembrane voltage n: number of pulses. |
θ |
Angle on the cell surface with respect to the direction of the applied field. |
θc
|
Critical angle. |
Σ0
|
Initial surface tension. |
Σel
|
Surface tension induced by the electric field. |
Σlys
|
Lysis tension. |
ɛm
|
Membrane dielectric constant. |
ρ |
Effective radius of the lipid hydrocarbon tail viewed as a cylinder. |
μ |
Lipid tail hydrophobic free energy per unit of area. |