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. 2000 Sep 12;97(20):10911–10916. doi: 10.1073/pnas.190204697

Figure 2.

Figure 2

Reconstitution of the TCR expressed by clone 37 into a murine T cell hybridoma. The TCR-transfected clone 37.33 expressing the chimeric human/mouse TCR was stained by the anti human TCRBV1 (BL37.2 mAb) and anti human CD4 (Middle). The untransfected mouse cell line 58α−β− served as negative control (Left). (Right) The expressions of TCR and CD4 in the parental human T cell clone 37. (B) Peptide-specific responsiveness of clone 37.33 was measured by using graded doses of peptide P3 (black bars) and monitoring IL-2 release and subsequent CTLL-2 cell proliferation. The irrelevant MBP peptide 13-32 (white bars) induced no IL-2 release. The TCR-transfected clone 37.33 remained stable in culture for over 2 yr without losing peptide specificity. All results are representative of three different experiments.