Figure 1. Generation of CA-MLCK mice expressing constitutively-activate MLCK.
(A) The CA-MLCK transgene construct. (B) Immunoblot of indicated organs from CA-MLCK Tg mice. (C) CA-MLCK, detected via the FLAG epitope tag (green), in jejunal epithelium. F-actin (red) and nuclei (blue) are shown. Bar, 10 μm.