Table 2.
The varieties studied, their typical alpha-acid levels, the start, end, and length of DNA sequences obtained from the twelve samples relative to ‘9418R’ (where base 1 of the sequences from the varieties in this study refers to base 2642 of AB047593), the nucleotide bases at which polymorphisms were observed, and the amino acids encoded by non-synonymous polymorphisms observed. ‘Saaz ×’ refers to a progeny of ‘Saaz’, and ‘Hallertau mf’ refers to ‘Hallertau mittelfrueh’. Triploid varieties are indicated by bold, all other varieties are diploid
| Nucleotide Base |
Amino acid residue |
||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Variety | Genbank accession | α-acid | Start | End | 351 | 723 | 762 | 779† | 780† | 874‡ | 909 | 1005 | 1084 | 232† | 264‡ |
| ‘9418R’ | AB047593 | – | 1 | 1269 | T | G | T | C | C | G | A | C | C | Ala | Ala |
| ‘Saaz ×’* | EU685798 | Low | 45 | 1238 | T/T | A/A | G/G | A/C | G/G | G/G | A/A | C/C | C/C | Ala/Glu | Ala |
| ‘Cascade’ | EU685789 | Low | 34 | 1238 | T/T | A/G | T/T | C/C | G/C | G/G | A/A | C/C | C/C | Ala | Ala |
| ‘Cluster’ | EU685791 | Low | 44 | 1230 | T/T | A/A | G/G | A/A | G/G | G/G | A/A | C/C | C/C | Glu | Ala |
| ‘Golding’ | EU685792 | Low | 44 | 1238 | T/T | A/G | G/T | C/C | G/C | G/G | A/A | C/C | C/C | Ala | Ala |
| ‘Hallertau mf’ | EU685790 | Low | 44 | 1239 | T/T | A/G | G/T | C/C | G/C | G/G | A/A | C/C | C/C | Ala | Ala |
| ‘Eastern Gold’ | EU685793 | High | 27 | 1238 | T/T | A/G | T/T | C/C | G/C | G/G | A/A | C/C | C/C | Ala | Ala |
| ‘Nugget’ | EU685794 | High | 44 | 1238 | T/T | A/A | G/G | A/A | G/G | G/G | A/A | C/C | C/C | Glu | Ala |
| ‘Opal’ | EU685795 | High | 45 | 1230 | T/T | A/G | G/T | C/C | G/C | G/G | A/A | C/C | C/C | Ala | Ala |
| ‘J78’* | EU685797 | High | 165 | 1269 | T/T | A/G | T/T | C/C | G/C | G/G | A/A | C/C | C/C | Ala | Ala |
| ‘Symphony’* | EU685799 | High | 177 | 1238 | C/T | A/A | G/T | A/C | G/G | G/T | A/C | G/C | C/T | Ala/Glu | Ala/Ser |
| ‘Ember’* | EU685800 | High | 45 | 1238 | T/T | A/G | G/T | A/C | G/C | G/G | A/A | C/C | C/C | Ala/Glu/Asp | Ala |
| ‘Victoria’ | EU685796 | High | 45 | 1238 | T/T | A/G | G/T | A/C | G/C | G/G | A/A | C/C | C/C | Ala/Glu/Asp | Ala |
* Indicates varieties in which the gene expression of VPS was studied.
† and ‡ indicate non-synonymous nucleotide polymorphisms, and the corresponding amino acid residue of the VPS protein encoded. Ala = alanine; Glu = glutamic acid; Asp = aspartic acid; and Ser = serine.