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. 2008 May 29;102(2):167–182. doi: 10.1093/aob/mcn084

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© The Author 2008. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

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Fig. 6. — Double fertilization in Tofieldia glutinosa. (A) Fertilization of the egg nucleus. The sperm nucleus is still visible within the egg nucleus and the tube nucleus is present in the adjacent synergid. (B) Fertilization of the secondary nucleus by the second sperm nucleus. The sperm nucleus is still visible (with nucleolus) within the secondary nucleus. (C) Second fertilization event involving two unfused polar nuclei and a sperm nucleus. All sections were stained with the DNA fluorochrome DAPI and visualized with differential interference contrast (DIC) and fluorescence optics. Images are composites of two micrographs of the same section (one with fluorescence and one with DIC). egg, Egg cell; pn, polar nucleus; sn, secondary nucleus; sp, sperm nucleus; syn, synergid cell; tn, tube nucleus. Scale bars = 10 µm.