Table 2.
Comparison of gene expression levels of 14 selected differentially expressed genes estimated from quantitative real-time RT−PCR and microarray analysis
| (A) Treatment × control | |||||
|---|---|---|---|---|---|
| Accession number | Functional annotation | Quantitative RT−PCR fold change |
Microarray fold change |
||
| HL vs. Control | |||||
| CD848486 | Putative ribosomal protein S12 (Oryza sativa) | 1·3 | 1·84 | ||
| CD848534 | Lipid transfer protein (A. thaliana) | 2·01 | 3 | ||
| HL + HT vs. Control | |||||
| CX947086 | Beta-tubulin (Zinnia elegans) | 1·49 | 2·04 | ||
| CD847616 | Aquaporin (Helianthus annuus) | 2·69 | 2·48 | ||
| CD847838 | Oxidoreductase NAD-binding domain-containing protein (A. thaliana) | 1·63 | 2·73 | ||
| (B) Treatment × Organ | |||||
| Quantitative RT–PCR fold change | Microarray fold change | ||||
| Accession number | Functional annotation | Leaves | Immature seeds | Leaves | Immature seeds |
| HL × Organ | |||||
| CD845834 | Cysteine protease (Aster tripolium) | 1·66 | 1 | 3·02 | −1·83 |
| CD845613 | ss-1,3-glucanase (Cichorium intybus × Cichorium endivia) | 2·91 | −1·18 | 4·44 | −1·62 |
| CD849757 | Expansin 3 (Zinnia elegans) | −2·48 | 1·34 | −1·33 | 2·08 |
| HT × Organ | |||||
| CD852690 | Cytochrome P450 family protein (A. thaliana) | −1·67 | 2·14 | −1·90 | 1·78 |
| CD850746 | Pectin acetylesterase (EC 3·1·1.-) precursor − (Vigna radiata) | 4·81 | −1·4 | 2·57 | −2·00 |
| CD849228 | Ferredoxin (Helianthus annuus) | 2·85 | 1·17 | 5·17 | −1·20 |
| HL + HT × Organ | |||||
| CD855563 | Unknown protein (A. thaliana) | −1·74 | 1·16 | −1·41 | 2·32 |
| CX943668 | Esterase/lipase/thioesterase family protein (A. thaliana) | 1·48 | −2·27 | 3·01 | −1·11 |
| CD846383 | Homeobox-leucine zipper protein (HD-ZIP protein 5) (A. thaliana) | 1·4 | 1·74 | 3·61 | 1·01 |
Fourteen cDNA clones exhibiting different expression patterns and belonging to different functional categories were subjected to real-time RT−PCR analysis to confirm the microarray results. The expression levels of the target genes derived from RT−PCR were normalized using β-actin as an internal control and the fold-change values were calculated using the 2−ΔΔCT method and represent changes of mRNA abundance between the compared treatments. Fold-change values derived from the microarray analysis were computed as the difference between normalized expression values of the compared treatments. Positive values denote up-regulation and negative values denote down-regulation.