. 2009 May 15;37(8):1682–1689. doi: 10.1124/dmd.109.026500

TABLE 2.

Effect of CPR on 1:1 CYP2C9/CYP2D6-mediated (S)-flurbiprofen metabolism for various mixing schemes

Addition of excess (20 pmol) CPR significantly (p < 0.05) increased the V_max ratios in Allmix1 and Allmix2 but not in SeqAdd1 and SeqAdd2. In SeqAdd3, a significant (p < 0.05) increase in V_max ratios was seen at 2.5 pmol of CYP2D6 only between the two CPR levels. No significant changes were observed in the K_m ratios upon addition of excess CPR in all systems. Single-factor analysis of variance was used for statistical comparisons. Data are the mean ± S.D.

	CPR	V_{max + 2D6}/V_max-control	K_{m + 2D6}/K_m-control	CPR	V_{max + 2D6}/V_max-control	K_{m + 2D6}/K_m-control
	pmol/incubation			pmol/incubation
SeqAdd1	10	0.60 ± 0.03	0.98 ± 0.23	20	0.61 ± 0.03	0.99 ± 0.12
SeqAdd2	10	0.69 ± 0.1	1.06 ± 0.27	20	0.78 ± 0.03	0.97 ± 0.26
SeqAdd3	10	0.52 ± 0.1	1.32 ± 0.65	20	0.69 ± 0.1	1.07 ± 0.32
Allmix1	10	0.54 ± 0.01	1.43 ± 0.56	20	0.71 ± 0.05	1.05 ± 0.11
Allmix2	10	0.56 ± 0.04	0.78 ± 0.10	20	0.8 ± 0.05	1.01 ± 0.27