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. 2009 Jul 2;10:51. doi: 10.1186/1471-2121-10-51

Table 2.

Primers and plasmids used for this study

primers
Designation Sequence (5' -> 3') or description use Reference(s)

AtCPSF30 5' AGATCTATGGAGGATGCTGATGGACTT Cloning of the AtCPSF30 protein-coding region This study

AtCPSF30 3' CCGGAGATCTATGTCGGGCCTCCATCGATC Cloning of the AtCPSF30 protein-coding region This study

C-ter 30 5' (m9) AGATCTGGAGCTGGGAGGGGTAGAAGTTTCCGTCAA Cloning of the AtCPSF30 C-terminal protein-coding region This study

N-ter 30 3' (m4) GGGCCCAGGTCCAGGAAGCTTTGCATGCCTGTACCGACA Cloning of the AtCPSF30 N-terminal protein-coding region This study

AtDcp2 5' CCGGAGATCTATGTCGGGCCTCCATCGATC3 Cloning of the AtDcp2 protein-coding region This study

AtDcp2 3' AATTGGGCCCCAAACTGACCAGTCAAGCTGAATTACCAG Cloning of the AtDcp2 protein-coding region This study

plasmids

designation source use Reference(s)

Salk clone U61209 ABRC; corresponds to At5g13570 Template for amplification of AtDcp2 sequences

pMAL-AtCPSF30 Hunt laboratory Template for amplification of AtCPSF30 sequences [14]

pMDC43C1-GFP::AtCPSF160, pMDC43C1-GFP::AtCPSF100, pMDC43C1-GFP-AtCPSF73(I) and GFP-AtCPSF73(II) Dr. Q. Q. Li (Miami University, Oxford, OH) Plasmids for the expression of GFP fused Arabidopsis CPSF factors 160, 100, 73CII and 73CI [30]

pGD RFP, pGD RFP-NLS, Dr. Michael Goodin (University of Kentucky, Lexington, KY) pGD RFP vector was used to express different CPSF 30 clones; pGD RFP-NLS was used as control in GFP fusion studies. [19]

pKLX80- AtZFP11-NLS::GFP, pKLX80-CoxII::GFP, pKLX80-ER::GFP Dinkins laboratory [20,22]