Designation |
Sequence (5' -> 3') or description |
use |
Reference(s) |
|
AtCPSF30 5' |
AGATCTATGGAGGATGCTGATGGACTT |
Cloning of the AtCPSF30 protein-coding region |
This study |
|
AtCPSF30 3' |
CCGGAGATCTATGTCGGGCCTCCATCGATC |
Cloning of the AtCPSF30 protein-coding region |
This study |
|
C-ter 30 5' (m9) |
AGATCTGGAGCTGGGAGGGGTAGAAGTTTCCGTCAA |
Cloning of the AtCPSF30 C-terminal protein-coding region |
This study |
|
N-ter 30 3' (m4) |
GGGCCCAGGTCCAGGAAGCTTTGCATGCCTGTACCGACA |
Cloning of the AtCPSF30 N-terminal protein-coding region |
This study |
|
AtDcp2 5' |
CCGGAGATCTATGTCGGGCCTCCATCGATC3 |
Cloning of the AtDcp2 protein-coding region |
This study |
|
AtDcp2 3' |
AATTGGGCCCCAAACTGACCAGTCAAGCTGAATTACCAG |
Cloning of the AtDcp2 protein-coding region |
This study |
|
|
plasmids |
|
|
|
designation |
source |
use |
Reference(s) |
|
Salk clone U61209
|
ABRC; corresponds to At5g13570 |
Template for amplification of AtDcp2 sequences |
|
|
pMAL-AtCPSF30 |
Hunt laboratory |
Template for amplification of AtCPSF30 sequences |
[14] |
|
pMDC43C1-GFP::AtCPSF160, pMDC43C1-GFP::AtCPSF100, pMDC43C1-GFP-AtCPSF73(I) and GFP-AtCPSF73(II) |
Dr. Q. Q. Li (Miami University, Oxford, OH) |
Plasmids for the expression of GFP fused Arabidopsis CPSF factors 160, 100, 73CII and 73CI |
[30] |
|
pGD RFP, pGD RFP-NLS, |
Dr. Michael Goodin (University of Kentucky, Lexington, KY) |
pGD RFP vector was used to express different CPSF 30 clones; pGD RFP-NLS was used as control in GFP fusion studies. |
[19] |
|
pKLX80- AtZFP11-NLS::GFP, pKLX80-CoxII::GFP, pKLX80-ER::GFP |
Dinkins laboratory |
|
[20,22] |