Figure 2.

Antagonist peptides induce phosphorylation of SLAP-130/FYB. AD10 cells (1 × 10⁷) were stimulated with antagonist (T102G) pulsed, agonist (PCC 88–104) pulsed, or unpulsed DCEK.ICAM APCs (1 × 10⁷). After 1 min of stimulation, cells were lysed, immunoprecipitated with anti-SLAP-130, ZAP-70, or LAT antibodies and analyzed by SDS/PAGE. Proteins were transferred to membranes and immunoblotted with antiphosphotyrosine antibody (Upper). Membranes were subsequently stripped and analyzed for SLAP-130, ZAP-70, or LAT protein (Lower). Results are representative of five similar experiments.