Table 1.
Chaperone/co-chaperonea | Elevation | Alterationb Depletion | Mutation | [PSI+] (Sup35p) | [URE3] (Ure2p) | [PIN+] (Rnq1p) | Reference |
Hsp70s | |||||||
Ssa1 | x | ⇑ | ⇓ | ? | 75, 85 | ||
Ssa1 | x | ⇓ | - | ? | 20, 84 | ||
Ssa1 | dominant neg. | ⇓ | ⇓ | ? | 58, 84 | ||
Ssa1 (only) | - | ⇓ | ? | 84 | |||
Ssa2 | x | ⇑ | - | ? | 52 | ||
Ssa2 | x | - | ⇓ | ? | 84 | ||
Ssa2 | dominant neg. | ⇓ | ⇓ | ? | 83S, 84 | ||
Ssa2 (only) | ⇑ | - | ? | 84 | |||
Ssa3 | x | ⇑ | ? | ? | 52 | ||
Ssa3 (only) | ⇑ | ⇓ | ? | 84 | |||
Ssa4 | x | ⇑ | ? | ? | 52 | ||
Ssa4 (only) | ⇓ | ⇓ | ? | 84 | |||
Hsp40s | |||||||
Ydj1 | x | - | ⇓ | ⇓d | 63–65 | ||
Ydj1 | x | - | ⇓e | - | 64, 65 | ||
Ydj1 | J-domain | ? | ⇓ | ? | 64, 65 | ||
Sis1 | x | - | - | ⇑ | 51, 64, 78 | ||
Sis1 | x | ⇓ | ⇓ | ⇓ | 64 | ||
Sis1 | G/F alteration | - | ? | ⇓ | 51, 72 | ||
Sis1 | J-domain | ? | ⇓ | ? | 65 | ||
Apj1 | x | ⇓f | - | - | 44, 64 | ||
Jjj1 | J-domain | ? | ⇓ | ? | 64 | ||
NEFs | |||||||
Fes1 | x | ⇑ | - | ? | 50 | ||
Fes1 | x | ⇓ | ⇓ | ? | 50, 67 | ||
Sse1 | x | ⇑ | ⇓ | ? | 67, 94 | ||
Sse1 | x | ⇓ | ⇓ | ? | 67, 94 | ||
TPRs | |||||||
Sti1 | x | ⇓g | ? | ? | 50, 94 | ||
Sti1 | x | ⇑ | ? | ? | 50 | ||
Cns1 | x | ⇓ | ? | ? | 50 | ||
Cpr7 | x | ⇑ | ? | ? | 50 | ||
Hsp82 (Hsp90) | x | ⇓ | ? | ? | 94 |
(only) means indicated Ssa protein is the only Ssa expressed in the cell.
x indicates alteration producing the effects. c⇑, enhances prion strength and/or stability; ⇓, weakens phenotype or eliminates prion; -, alteration has no effect; ?, unknown.
Cures cells of some [PIN+] variants, not others.
Cures cells of some [PSI+PS] variants, not others (see text).