Table 1.

Effects of altered chaperones on prion phenotypes

Chaperone/co-chaperonea	Elevation	Alterationb Depletion	Mutation	[PSI+] (Sup35p)	[URE3] (Ure2p)	[PIN+] (Rnq1p)	Reference
Hsp70s
Ssa1	x			⇑	⇓	?	75, 85
Ssa1		x		⇓	-	?	20, 84
Ssa1			dominant neg.	⇓	⇓	?	58, 84
Ssa1 (only)				-	⇓	?	84
Ssa2	x			⇑	-	?	52
Ssa2		x		-	⇓	?	84
Ssa2			dominant neg.	⇓	⇓	?	83S, 84
Ssa2 (only)				⇑	-	?	84
Ssa3	x			⇑	?	?	52
Ssa3 (only)				⇑	⇓	?	84
Ssa4	x			⇑	?	?	52
Ssa4 (only)				⇓	⇓	?	84
Hsp40s
Ydj1	x			-	⇓	⇓d	63–65
Ydj1		x		-	⇓e	-	64, 65
Ydj1			J-domain	?	⇓	?	64, 65
Sis1	x			-	-	⇑	51, 64, 78
Sis1		x		⇓	⇓	⇓	64
Sis1			G/F alteration	-	?	⇓	51, 72
Sis1			J-domain	?	⇓	?	65
Apj1	x			⇓f	-	-	44, 64
Jjj1			J-domain	?	⇓	?	64
NEFs
Fes1	x			⇑	-	?	50
Fes1	x			⇓	⇓	?	50, 67
Sse1	x			⇑	⇓	?	67, 94
Sse1		x		⇓	⇓	?	67, 94
TPRs
Sti1	x			⇓g	?	?	50, 94
Sti1		x		⇑	?	?	50
Cns1	x			⇓	?	?	50
Cpr7		x		⇑	?	?	50
Hsp82 (Hsp90)	x			⇓	?	?	94

^a(only) means indicated Ssa protein is the only Ssa expressed in the cell.

^bx indicates alteration producing the effects. ^c⇑, enhances prion strength and/or stability; ⇓, weakens phenotype or eliminates prion; -, alteration has no effect; ?, unknown.

^dCures cells of some [PIN⁺] variants, not others.

^eydj1Δ is compatible with [URE3] in one study,⁶⁴ lethal with Ure2p depletion in another.⁶⁵

^fCures cells of some [PSI⁺_PS] variants, not others (see text).

^gimproves [PSI⁺] in SSA1-21 (Hsp70 dominant negative) mutant,⁵⁰ inhibits induction of [PSI⁺] by Sup35 NM overexpression.⁹⁴