Table 5.
Survey of Activities on Benzyl Alcohols and Benzaldehydesa
| Enzyme | Benzyl alcohol | p-Methoxy- benzyl alcohol | Benzaldehyde | p-Methoxy- benzaldehyde |
|---|---|---|---|---|
| V/Et (s−1) | ||||
| SceADH1 | 0.012 | 0.058 | 0.91 | 0.075 |
| ScbADH1 | 0.011 | 0.11 | 0.52 | 0.071 |
| SceADH2 | 1.9 | 0.87 | 12 | 0.86 |
| Km (mM) | ||||
| SceADH1 | 34 | 11 | 33 | 2.9 |
| ScbADH1 | 24 | 22 | 23 | 3.4 |
| SceADH2 | 49 | 6.9 | 14 | 5.9 |
| V/Km (M−1s−1) | ||||
| SceADH1 | 0.32 | 5.3 | 27 | 26 |
| ScbADH1 | 0.48 | 4.5 | 23 | 21 |
| SceADH2 | 38 | 130 | 860 | 150 |
| Highest Concentrations of Alcohols and Aldehydes (mM) | ||||
| SceADH1 | 25 | 15 | 32 | 5 |
| ScbADH1 | 25 | 15 | 32 | 5 |
| SceADH2 | 25 | 35 | 55 | 5 |
a
Assays used 2 mM NAD+ and different concentrations of NADH (250 μM NADH for benzaldehyde and 100 μM NADH for p-methoxybenzaldehyde). V is V1 for alcohols and V2 for aldehydes. Km is the Kb for the alcohols and Kp for the aldehydes. The kinetic constants are apparent because the concentrations of coenzymes were not saturating.