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. Author manuscript; available in PMC: 2009 Jul 20.
Published in final edited form as: Int J Parasitol. 2007 Apr 19;37(11):1233–1241. doi: 10.1016/j.ijpara.2007.03.013

Fig. 2.

Fig. 2

Reactivity of human immune sera and stage-specific expression of Pf34. (A) The recombinant proteins Pf34-A (lane A), Pf34-B (lane B) and Pf34-C (lane C) and glutathione-S-transferase alone (lane D) were resolved under denaturing conditions. Shown are Coomassie-stained samples and those immunoblotted with human immune sera from endemic areas of Vietnam and Papua New Guinea, and non-immune sera from Australia. (B) Stage-specific expression of Pf34. Synchronised parasite culture was sampled at various time points (indicated in hours) and lysates resolved by SDS-PAGE. All samples were immunoblotted with antisera raised against Pf34 fragments A, C and RAMA. Anti-GRP(BiP) sera was used as a loading control. Pf34 expression is not convincingly demonstrable at 22–28 h.